DataEntryID 42
    General information
      Manuscript title: A systems biology approach to investigate the effect of pH-induced gene regulation on solvent production by Clostridium acetobutylicum in continuous culture.
      PubMed ID: http://www.ncbi.nlm.nih.gov/pubmed/21247470
      Journal: BMC Systems Biology
      Year: 2011
      Authors: Sylvia Haus, Sara Jabbari, Thomas Millat, Holger Janssen, Ralf-Jörg Fischer, Hubert Bahl, John R King, Olaf Wolkenhauer
      Affiliations: University of Rostock, Institute of Computer Science, Department of Systems Biology and Bioinformatics
      Keywords: Clostridium acetobutylicum, continuous culture, effect of pH, solvent production
      Full text article: https://kimosys.org/rails/active_storage/blobs/eyJfcmFpbHMiOnsibWVzc2FnZSI6IkJBaHBBbXdFIiwiZXhwIjpudWxsLCJwdXIiOiJibG9iX2lkIn19--78abd31245010abbb38ad36f5ed962affbb64ec9/Haus_2011.pdf
      Project name: not specified

    Experiment description
      Organism: Clostridium acetobutylicum
      Strain: ATCC824
      Data type: time-series data of metabolites
      Data units: mM
      Execution date: not specified

    Experimental details
      Temperature (°C): 37.0
      pH: 5.7 and 4.5
      Carbon source: glucose
      Culture mode: chemostat
      Process condition: anaerobic
      Dilution rate (h⁻¹): 0.1
      Working volume: 1.0 L
      Biomass concentration (g/L): not specified
      Medium composition: 4% (wt/vol) glucose in the supplying medium
      General protocol information: Sampling Method: 2 ml of C. acetobutylicum cell suspensions were collected and quickly sedimented at 16,000 g for 30s at 4°C in a benchtop centrifuge.; Quenching: —; Extraction list: not used; Analysis list: GC-MS;
      Methods description: Separation took place in a Chromosorb 101 column (length, 2 m; 80 to 100 mesh) at 155 to 197°C (rate of temperature increase, 9°C/min) with N2 as the carrier gas (flow rate, 30 ml/min).  Injector temperature was 195°C, and the detector temperature was 230°C. Signal analysis was performed by using the program Maestro II.
      Data file: http://kimosys.org/repository/42/download?parameter=1131; 
      Alternative formats: http://kimosys.org/repository/42/attached_files/26/download; http://kimosys.org/repository/42/attached_files/25/download; http://kimosys.org/repository/42/attached_files/24/download; http://kimosys.org/repository/42/attached_files/23/download; 

    Submission and curation
      Entered by: Administrator KiMoSys
      Created: 2013-05-03 10:45:33 UTC
      Updated: 2020-04-24 16:10:31 UTC
      Version: 0
      Status: (reviewed) 2013-12-06 17:17:38 UTC
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      Downloads: 215