DataEntryID 41
    General information
      Manuscript title: Multiple High-Throughput Analyses Monitor the Response of E. coli to Perturbations.
      PubMed ID: http://www.ncbi.nlm.nih.gov/pubmed/17379776
      Journal: Science
      Year: 2007
      Authors: Nobuyoshi Ishii, Kenji Nakahigashi, Tomoya Baba, Martin Robert, Tomoyoshi Soga, Akio Kanai, Takashi Hirasawa, Miki Naba, Kenta Hirai, Aminul Hoque, Pei Yee Ho, Yuji Kakazu, Kaori Sugawara, Saori Igarashi, Satoshi Harada, Takeshi Masuda, Naoyuki Sugiyama, Takashi Togashi, Miki Hasegawa, Yuki Takai, Katsuyuki Yugi, Kazuharu Arakawa, Nayuta Iwata, Yoshihiro Toya, Yoichi Nakayama, Takaaki Nishioka, Kazuyuki Shimizu, Hirotada Mori, Masaru Tomita
      Affiliations: Institute for Advanced Biosciences, Keio University
      Keywords: Escheirchia coli, perturbations response, chemostat, single knockouts
      Full text article: no file uploaded
      Project name: not specified

    Experiment description
      Organism: Escherichia coli
      Strain: WT K-12 BW25113 and mutants
      Data type: metabolites at steady-state
      Data units: mM
      Execution date: not specified

    Experimental details
      Temperature (°C): 37.0
      pH: 7.0
      Carbon source: glucose
      Culture mode: chemostat
      Process condition: aerobic
      Dilution rate (h⁻¹): 0.1, 0.2, 0.4, 0.5 and 0.7
      Working volume: 1.0 L
      Biomass concentration (g/L): See Supplementary files of the original article (Tabel S3).
      Medium composition: Synthetic medium: 48mM Na2HPO4, 22mM KH2PO4, 10mM NaCl, 45mM (NH4)2SO4, 4g/L glucose, 1mM MgSO4, 1mg/L thiamin.HCL,  5.6 mg/L CaCl2, 8 mg/L FeCl3, 1 mg/L MnCl2.4H2O, 1.7 mg/L ZnCl2, 0.43 mg/L CuCl2.2H2O, 0.6 mg/L CoCl2.2H2O and 0.6 mg/L Na2MoO4.2H2O
      General protocol information: Sampling Method: Aliquots of 10 mL were withdrawn at OD600 0.85; Quenching: Cells were filter (0.45-µm), washed with 10 mL of Milli-Q water and plunged into 1 mL of methanol containing 10µL of 0.56 mM PIPES; Extraction list: chloroform; Analysis list: CE-MS;
      Methods description: Metabolites were quantified using peak areas obtained from measurements of chemical standards mixtures that were analyzed in parallel with experimental samples. See more details in the original article.
      Data file: http://kimosys.org/repository/41/download?parameter=1130; 
      Alternative formats: no files uploaded

    Submission and curation
      Entered by: Administrator KiMoSys
      Created: 2013-05-02 17:35:25 UTC
      Updated: 2020-04-24 16:10:31 UTC
      Version: 1
      Status: (reviewed) 2013-12-06 17:17:38 UTC
      Views: 615
      Downloads: 57