DataEntryID 105
    General information
      Manuscript title: Metabolic flux analysis for a ppc mutant Escherichia coli based on 13C-labelling experiments together with enzyme activity assays and intracellular metabolite measurements.
      PubMed ID: http://www.ncbi.nlm.nih.gov/pubmed/15158257
      Journal: FEMS Microbiology Letters
      Year: 2004
      Authors: Lifeng Peng, Marcos J. Arauzo-Bravo, Kazuyuki Shimizu
      Affiliations: Department of Biochemical Engineering and Science, Kyushu Institute of Technology, Iizuka, Fukuoka 820-8502, Japan
      Keywords: ppc mutant Escherichia coli, 2-Dimensional nuclear magnetic resonance, Gas chromatography-mass spectrometry, Metabolic flux analysis
      Full text article: https://kimosys.org/rails/active_storage/blobs/eyJfcmFpbHMiOnsibWVzc2FnZSI6IkJBaHBBc29FIiwiZXhwIjpudWxsLCJwdXIiOiJibG9iX2lkIn19--4951d1618a323e2bfcb9ec43d099ee73ec3467c7/Peng_2004.pdf
      Project name: not specified

    Experiment description
      Organism: Escherichia coli
      Strain: BW25113 and ppc mutant
      Data type: flux measurements
      Data units: (mmol/g-dry cell weight/h)
      Execution date: not specified

    Experimental details
      Temperature (°C): 37
      pH: 7.0
      Carbon source: glucose
      Culture mode: chemostat
      Process condition: aerobic
      Dilution rate (h⁻¹): 0.2
      Working volume: 1.0 L
      Biomass concentration (g/L): see worksheet.
      Medium composition: M9 minimal medium as described previously [3].
      General protocol information: Type analysis list: 13C constrained MFA; Platform list: NMR, GC-MS;
      Methods description: The fluxes of Pgi, Pyk, CS were chosen as the net free fluxes, and 11 exchange fluxes (Pgi, Eno, Rpi, Rpe, Tkt1, Tkt2, Tal, ICDH, Fum, Mdh, Ppc/Pck) were considered. In both cases, the free fluxes were obtained by minimizing the error criterion originating from the weighted residuals of the metabolite balances as well as the weighted residuals between the estimated and the measured GC-MS and 2D NMR signals [1]. The modified minimization algorithm [2] was used to compute the intracellular fluxes. A set of intracellular fluxes was then determined as the best fit to the experimentally determined data using a parameter fitting approach.
--------------------References---------------
[1] Dauner M. Bailey, J. Sauer U. (2001). Biotechnol. Bioeng. 76, 132–143. http://doi.org/ffkpm2
[2] Araúzo-Bravo M.J., Shimizu K. (2003). J. Biotechnol. 105, 117–133. http://doi.org/cpnw8k                                                                                                                                                                                                                             [3] Zhao J., Shimizu K. (2002). J. Biotechnol. 101, 101–117. http://doi.org/cdjhrn                                                                                                                                                                                                                        
      Data file: http://kimosys.org/repository/105/download?parameter=1225; 
      Alternative formats: no files uploaded

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      Entered by: Administrator KiMoSys
      Created: 2018-07-21 14:36:12 UTC
      Updated: 2020-04-24 16:10:36 UTC
      Version: 2
      Status: (reviewed) 2018-07-21 14:43:53 UTC
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